In plain English
PE-22-28 is a seven-amino-acid peptide based on spadin, a natural peptide that blocks a potassium channel linked to depression. In animals it worked as an antidepressant within days rather than weeks, and it also boosted new nerve cell growth.
What it's used for
- Mood and depression
- Stress resilience
- Memory and new nerve cell growth
Benefits
In animal studies, PE-22-28 produced antidepressant effects within four days, increased new nerve cell growth and improved memory. Users report better mood, calm and motivation.
How it works
In simple terms: it switches off a channel that keeps mood-related neurons quiet.
It blocks TREK-1, a potassium channel. Animals without TREK-1 are resistant to depression, so blocking it is thought to lift mood and support neurogenesis.
Development history
- 2010: Spadin discovered in France by Jean Mazella’s group.
- 2015: Shorter, more stable analogs including PE-22-28 described.
Typical protocols
Doses vary; these ranges are what users commonly report.
| Use | Dose | Frequency | Length |
|---|---|---|---|
| Mood | 200–400 mcg | Daily | 2–4 weeks |
Cycling
Commonly run for 2–4 weeks, then a break.
Administration options
Nasal spray or subcutaneous injection.
Reconstitution and dosing calculator
Usually comes in 5 mg or 10 mg vials, mixed with 2 mL of bacteriostatic water (or saline for nasal use).
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This tool does arithmetic only. It does not choose a dose or confirm that any substance is right for you. Double-check every result against your syringe and your clinician’s instructions.
Side effects and safety
- Users report few side effects; headache and nasal irritation are mentioned.
- No formal safety data in people.
- Anyone being treated for depression should talk to their doctor before adding anything new.
- Avoid during pregnancy and breastfeeding.
Common stacks
- Selank: for calm.
- Semax: for focus.
Storage
Powder: refrigerate or freeze. After mixing: refrigerate at 2–8 °C and use within about 3 weeks.
Compare with
- Selank: a calming nasal peptide used clinically in Russia.
- Semax: more focused on attention and energy.
Frequently asked questions
Is it like an antidepressant medicine?
It works through a different target (TREK-1). In animals it acted faster, but it has not been tested in people.
References
- Djillani A, et al. Shortened spadin analogs display better TREK-1 inhibition, in vivo stability and antidepressant activity. Front Pharmacol. 2017;8:643.